Human Amyloid beta (aa1-42) Quantikine ELISA Kit
Human Amyloid beta (aa1-42) Quantikine ELISA Kit
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Human Amyloid beta (aa1-42) Quantikine ELISA Kit Summary
貨號：DAB142 規(guī)格：96T
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Tissue Lysates (10 uL), Cerebrospinal Fluid (10 uL)
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid)
Specificity
Natural human Amyloid beta (aa1-42).
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Amyloid beta (aa1-42) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Amyloid beta (aa1-42) in cell culture supernates, tissue lysates, and cerebrospinal fluid. It contains synthetic human Amyloid beta (aa1-42) and antibodies raised against the synthetic peptide. Results obtained for naturally occurring human Amyloid beta
(aa1-42) showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-42).
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid
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|
Intra-Assay Precision
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Inter-Assay Precision
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|
Sample
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1
|
2
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3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
46.3
|
124
|
264
|
48.9
|
136
|
274
|
|
Standard Deviation
|
2.46
|
4.4
|
9.38
|
3.48
|
9.02
|
16.1
|
|
CV%
|
5.3
|
3.5
|
3.6
|
7.1
|
6.6
|
5.9
|
Recovery
The recovery of human Amyloid beta (aa1-42) spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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|
Cell Culture Media (n=4)
|
97
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82-114
|
|
Cell Lysis Buffer (n=2)
|
92
|
90-94
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|
Cerebrospinal Fluid (n=4)
|
107
|
95-120
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Linearity
To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta (aa1-42) were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.
