熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
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熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統(tǒng)一客服熱線：
0755-28715175
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貨號：DPI00
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
The recovery of SLPI was determined in various matrices by mixing high dose samples with low dose samples in ratios of 1:2, 1:1, and 2:1.
Human SLPI Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Urine (20 uL)
Sensitivity
25 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human SLPI
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human SLPI Immunoassay is a 4.5 hour solid phase ELISA designed to measure SLPI in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human SLPI and antibodies raised against the recombinant factor. The kit has been shown to accurately quantitate recombinant human SLPI. Results obtained using natural human SLPI showed linear curves that were parallel to the curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human SLPI.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
109
693
2026
188
1174
2157
Standard Deviation
8.7
37.1
84.1
15.1
57.5
123
CV%
8
5.4
4.2
8
4.9
5.7
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=5)
94
84-105
Citrate Plasma (n=10)
97
92-102
EDTA Plasma (n=10)
96
87-103
Heparin Plasma (n=10)
106
98-116
Serum (n=10)
98
89-104
Urine (n=25)
98
92-103
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